Lab on a chip-科学家开发出新一代DNA分析技术
Lab on a chip：科学家开发出新一代DNA分析技术
2011/05/23 11:51:17
韩国教育科技部和韩国研究财团5月5日报道，韩国西江大学赵圭峰（音译）教授领导的研究小组，近日成功开发了可在短时间内正确分析遗传基因（DNA）信息的下一代遗传基因分析技术。
该技术可将遗传基因分子链条长度拉长为接近理论长度90%的19微米，并将其放入纳米通道进行分析，正确度比以往提高了1.5倍。研究小组称，为迅速分析大量遗传基因信息，必须最大限度地拉长遗传基因链条，此次研究结果实现了这一目标，可迅速而准确地分析大量遗传基因信息。
该项研究成果已在英国皇家化学会《芯片实验室》（Lab on a chip）上作为封面论文刊登。
推荐原文出处：
Lab on a chip DOI: 10.1039/C0LC00680G
Nanochannel confinement: DNA stretch approaching full contour length
Yoori Kim, Ki Seok Kim, Kristy L. Kounovsky, Rakwoo Chang, Gun Young Jung, Juan J. dePablo, Kyubong Jo and David C. Schwartz
Fully stretched DNA molecules are becoming a fundamental component of new systems for comprehensive genome analysis. Among a number of approaches for elongating DNA molecules, nanofluidic molecular confinement has received enormous attentions from physical and biological communities for the last several years. Here we demonstrate a well-optimized condition that a DNA molecule can stretch almost to its full contour length: the average stretch is 19.1 μm ± 1.1 μm for YOYO-1 stained λ DNA (21.8 μm contour length) in 250 nm × 400 nm channel, which is the longest stretch value ever reported in any nanochannels or nanoslits. In addition, based on Odijk’s polymer physics theory, we interpret our experimental findings as a function of channel dimensions and ionic strengths. Furthermore, we develop a Monte Carlo simulation approach using a primitive model for the rigorous understanding of DNA confinement effects. Collectively, we present a more complete understanding of nanochannel confined DNA stretching via the comparisons to computer simulation results and Odijk’s polymer physics theory.